Hotspot

rpoC2

LSC · 16717-20826 · 4110 bp

Back to Caryopteris

Pi 0.00122

MarkerSeek score 54.70

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4110
variable_sites: 10
pi: 0.00122
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CCCAATTAGTTCTCCCGGTG	AGGTGTTAGAAGTGCGTTCG	55.0	50.0	58.0	58.2	299 bp	1.0	79.0
rpoC2_p2	CCCAATTAGTTCTCCCGGTG	GGTGTTAGAAGTGCGTTCGA	55.0	50.0	58.0	58.2	298 bp	1.0	79.0
rpoC2_p3	CCCAATTAGTTCTCCCGGTG	AACCCAGGGTCTTCCAAAAG	55.0	50.0	58.0	57.6	325 bp	1.0	77.6
rpoC2_p4	ACCCAATTAGTTCTCCCGGT	AGGTGTTAGAAGTGCGTTCG	50.0	50.0	58.3	58.2	300 bp	1.0	76.6
rpoC2_p5	ACCCAATTAGTTCTCCCGGT	GGTGTTAGAAGTGCGTTCGA	50.0	50.0	58.3	58.2	299 bp	1.0	76.6