Hotspot

rpoC1

LSC · 20993-23820 · 2828 bp

Back to Caryopteris

Pi 0.00124

MarkerSeek score 57.00

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2828
variable_sites: 7
pi: 0.00124
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCTCGAACTATGGGCCTAGT	AGGAAGGAAGCTTAATCGGA	50.0	45.0	57.2	55.9	2936-2941 bp	1.0	57.8
rpoC1_p2	TGGGCCTAGTTGAAGTGAAG	AGGAAGGAAGCTTAATCGGA	50.0	45.0	57.1	55.9	2926-2931 bp	1.0	56.9
rpoC1_p3	TCTCGAACTATGGGCCTAGT	AGGAAGGAAGCTTAATCGGAA	50.0	42.9	57.2	56.7	2936-2941 bp	1.0	56.1
rpoC1_p4	GGGCCTAGTTGAAGTGAAGAG	GCGAGAACTACGATCTTTAGCT	52.4	45.5	58.0	58.0	2995-3000 bp	1.0	55.8
rpoC1_p5	GGGCCTAGTTGAAGTGAAGAG	AGGAAGGAAGCTTAATCGGA	52.4	45.0	58.0	55.9	2925-2930 bp	1.0	55.6