Hotspot

trnW-CCA-trnP-UGG

LSC · 65861-66046 · 186 bp

Back to Caryopteris

Pi 0.0148

MarkerSeek score 46.50

Primer available yes

Label trnW-CCA-trnP-UGG

Feature Metadata

label_name: trnW-CCA-trnP-UGG
feature_type: igs
region: LSC
length_bp: 186
variable_sites: 5
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-trnP-UGG_p1	GCGTTCTACCGAACTGAACT	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	256-273 bp	1.0	84.1
trnW-CCA-trnP-UGG_p2	ACCTACGACATCGGGTTTTG	CTTGGTAGCGCGTTTGTTTT	50.0	45.0	57.9	57.9	283-300 bp	1.0	83.8
trnW-CCA-trnP-UGG_p3	GCGTTCTACCGAACTGAACT	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	255-272 bp	1.0	83.7
trnW-CCA-trnP-UGG_p4	GCGTTCTACCGAACTGAACT	CGCGTTTGTTTTGGGTACAA	50.0	45.0	57.9	57.8	248-265 bp	1.0	83.6
trnW-CCA-trnP-UGG_p5	ACCTACGACATCGGGTTTTG	TTGGTAGCGCGTTTGTTTTG	50.0	45.0	57.9	58.2	282-299 bp	1.0	83.3