Hotspot

psbM-trnD-GUC

LSC · 29852-30374 · 523 bp

Back to Caryopteris

Pi 0.00765

MarkerSeek score 50.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 523
variable_sites: 8
pi: 0.00765
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGCGGTAGGAACTAGAATGA	TCTTTCCCCGGGATTGTAGT	47.6	50.0	58.0	58.0	667 bp	1.0	72.4
psbM-trnD-GUC_p2	AGAAAGGCGGTAGGAACTAGA	TCTTTCCCCGGGATTGTAGT	47.6	50.0	57.9	58.0	671 bp	1.0	71.9
psbM-trnD-GUC_p3	AGTAGAAAGGCGGTAGGAACT	TCTTTCCCCGGGATTGTAGT	47.6	50.0	58.2	58.0	674 bp	1.0	71.2
psbM-trnD-GUC_p4	AGGCGGTAGGAACTAGAATGA	TAGATCCCTTCTTTCCCCGG	47.6	55.0	58.0	58.2	676 bp	1.0	71.1
psbM-trnD-GUC_p5	AGAAAGGCGGTAGGAACTAGA	TAGATCCCTTCTTTCCCCGG	47.6	55.0	57.9	58.2	680 bp	1.0	70.6