Hotspot

atpH-atpI

LSC · 13794-14804 · 1011 bp

Back to Caryopteris

Pi 0.00247

MarkerSeek score 51.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1011
variable_sites: 5
pi: 0.00247
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGCAGTTCCTTGACCAACT	CCCGTCATGTTCCTTGGATT	50.0	50.0	58.0	57.9	1207-1227 bp	1.0	79.1
atpH-atpI_p2	GTTCCTTGACCAACTCCAGG	CCCGTCATGTTCCTTGGATT	55.0	50.0	58.1	57.9	1202-1222 bp	1.0	78.5
atpH-atpI_p3	AACGGAAGCAGCAGAAATCA	CCCGTCATGTTCCTTGGATT	45.0	50.0	58.1	57.9	1143-1163 bp	1.0	78.5
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	CCCGTCATGTTCCTTGGATT	45.0	50.0	57.9	57.9	1161-1181 bp	1.0	78.1
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	CCCGTCATGTTCCTTGGATT	50.0	50.0	57.8	57.9	1160-1180 bp	1.0	77.6