Hotspot

trnS-GCU-trnG-UCC

LSC · 8572-9233 · 662 bp

Back to Caryopteris

Pi 0.00389

MarkerSeek score 41.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 5
pi: 0.00389
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	TCCACTCAGCCATCTCTCC	AACCGAAAGACCCTTTAACT	57.9	40.0	58.1	54.5	739-751 bp	1.0	45.8
trnS-GCU-trnG-UCC_p2	TCCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	57.9	38.1	58.1	55.5	703-715 bp	1.0	45.8
trnS-GCU-trnG-UCC_p3	TCCACTCAGCCATCTCTCC	AGAACGAATCACACTTTTACCAC	57.9	39.1	58.1	57.3	706-718 bp	1.0	44.7
trnS-GCU-trnG-UCC_p4	TCCACTCAGCCATCTCTCC	GAACGAATCACACTTTTACCACT	57.9	39.1	58.1	57.3	705-717 bp	1.0	44.7
trnS-GCU-trnG-UCC_p5	TCCACTCAGCCATCTCTCC	AAACCGAAAGACCCTTTAACT	57.9	38.1	58.1	55.3	740-752 bp	1.0	44.6