Hotspot

rps16-trnQ-UUG

LSC · 6221-7453 · 1233 bp

Back to Caryopteris

Pi 0.00295

MarkerSeek score 65.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1233
variable_sites: 7
pi: 0.00295
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1282-1364 bp	1.0	83.2
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1224-1306 bp	1.0	82.7
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.4	58.0	1296-1378 bp	1.0	81.4
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1238-1320 bp	1.0	80.9
rps16-trnQ-UUG_p5	AAGAGCACCCCCATTCCTAG	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.8	58.0	1351-1433 bp	1.0	78.1