Hotspot

trnK-UUU-rps16

LSC · 4195-5056 · 862 bp

Back to Caryopteris

Pi 0.0058

MarkerSeek score 62.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 862
variable_sites: 10
pi: 0.0058
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	CCACAGGAACCGTTCAGAAT	50.0	50.0	58.2	57.8	976-988 bp	1.0	78.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	CCACAGGAACCGTTCAGAAT	50.0	50.0	58.2	57.8	975-987 bp	1.0	78.0
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	CCACAGGAACCGTTCAGAAT	50.0	50.0	58.3	57.8	990-1002 bp	1.0	77.2
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	CCACAGGAACCGTTCAGAAT	50.0	50.0	58.3	57.8	985-997 bp	1.0	77.1
trnK-UUU-rps16_p5	TACCGTTGAGTTAGCAACCC	CCACAGGAACCGTTCAGAAT	50.0	50.0	57.5	57.8	962-974 bp	1.0	75.7