Hotspot

ycf1

Genome · 125248-130773 · 5526 bp

Back to Caryodaphnopsis

Pi 0.147

MarkerSeek score 74.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 5526
variable_sites: 1508
pi: 0.147
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCCGAATATGTTTCTGCGA	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	58.0	58.0	527 bp	0.5	60.8
ycf1_p2	TGCCGAATATGTTTCTGCGA	GTTCTCCCCGAAAACAACCT	45.0	50.0	58.0	58.0	520 bp	0.5	60.8
ycf1_p3	TACGACGTCGATTGACCCTA	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	58.0	58.0	1126 bp	0.5	60.6
ycf1_p4	TGCCGAATATGTTTCTGCGA	TTTCTGTCTGAAACCCTGGC	45.0	50.0	58.0	58.0	648 bp	0.5	60.6
ycf1_p5	TACGACGTCGATTGACCCTA	TTTCTGTCTGAAACCCTGGC	50.0	50.0	58.0	58.0	1247 bp	0.5	60.4