Hotspot

ndhD

Genome · 116904-118439 · 1536 bp

Back to Caryodaphnopsis

Pi 0.18

MarkerSeek score 73.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1536
variable_sites: 512
pi: 0.18
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGTGTGAATTCGGCAATTG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2102 bp	0.5	60.6
ndhD_p2	GAGGCATGGGGGTCTTATTG	TCTGCTCCAAGAACAGAGGA	55.0	50.0	58.0	58.0	2209 bp	0.5	60.5
ndhD_p3	GGGTGTGAATTCGGCAATTG	AGATGTGAATCCGCTTGTCC	50.0	50.0	58.0	58.0	2066 bp	0.5	60.5
ndhD_p4	GGGTGTGAATTCGGCAATTG	CTATTGGGCCGGCTATTGTT	50.0	50.0	58.0	58.0	2577 bp	0.5	60.4
ndhD_p5	GAGGCATGGGGGTCTTATTG	CTATTGGGCCGGCTATTGTT	55.0	50.0	58.0	58.0	2684 bp	0.5	60.3