Hotspot

ndhA

Genome · 121123-123308 · 2186 bp

Back to Caryodaphnopsis

Pi 0.226

MarkerSeek score 74.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: Genome
length_bp: 2186
variable_sites: 912
pi: 0.226
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AACCCAGTCACCATAGGGAA	AAAGCCTTCTCCCACCTTTG	50.0	50.0	57.9	58.0	2532-2550 bp	0.5	59.8
ndhA_p2	AACCCAGTCACCATAGGGAA	CAATGTTACGAGCTTCCGGA	50.0	50.0	57.9	58.0	2806-2824 bp	0.5	59.8
ndhA_p3	AACCCAGTCACCATAGGGAA	TGTGAGAGTAGAAGCCCCAA	50.0	50.0	57.9	58.0	2490-2508 bp	0.5	59.7
ndhA_p4	AACCCAGTCACCATAGGGAA	AGTTGAAGGAGTGGGCATTG	50.0	50.0	57.9	58.1	2865-2883 bp	0.5	59.2
ndhA_p5	ACTCGACGCAATTACCACAA	AAAGCCTTCTCCCACCTTTG	45.0	50.0	57.8	58.0	2846-2864 bp	0.5	59.0