Hotspot

ccsA

Genome · 115727-116677 · 951 bp

Back to Caryodaphnopsis

Pi 0.237

MarkerSeek score 70.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 951
variable_sites: 416
pi: 0.237
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	1486 bp	0.5	60.7
ccsA_p2	TGAAATCGGTAGACACGCTG	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	1485 bp	0.5	60.7
ccsA_p3	GTGAAATCGGTAGACACGCT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1710 bp	0.5	60.6
ccsA_p4	TGAAATCGGTAGACACGCTG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	1709 bp	0.5	60.6
ccsA_p5	GTGAAATCGGTAGACACGCT	CTCGATGGCTTCTCTTGCAT	50.0	50.0	58.0	58.0	1708 bp	0.5	60.3