Hotspot

rpl32-trnL-UAG

Genome · 114372-115547 · 1176 bp

Back to Caryodaphnopsis

Pi 0.204

MarkerSeek score 75.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 1176
variable_sites: 444
pi: 0.204
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGAACAAACAGAAGTCCCA	ACAGAAAAAGGCAGCCATCA	50.0	45.0	58.0	58.0	2117-2141 bp	0.5	60.7
rpl32-trnL-UAG_p2	CGGAACAAACAGAAGTCCCA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1814-1838 bp	0.5	60.7
rpl32-trnL-UAG_p3	CGGAACAAACAGAAGTCCCA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1815-1839 bp	0.5	60.7
rpl32-trnL-UAG_p4	ACAAACAGAAGTCCCAACCC	ACAGAAAAAGGCAGCCATCA	50.0	45.0	57.9	58.0	2113-2137 bp	0.5	60.5
rpl32-trnL-UAG_p5	ACAAACAGAAGTCCCAACCC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1810-1834 bp	0.5	60.5