Hotspot

ndhF

SSC · 116183-118408 · 2226 bp

Back to Carya

Pi 0.0024
MarkerSeek score 33.60
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2226
variable_sites
18
pi
0.0024
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
33.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACTTCCGAAACAAAGGGGAC TCAATGGTAACTTGGTCATCTCA 50.0 39.1 58.0 57.5 2963-2980 bp 0.9 52.9
ndhF_p2 ACTTCCGAAACAAAGGGGAC CAATGGTAACTTGGTCATCTCA 50.0 40.9 58.0 56.1 2962-2979 bp 0.9 49.4
ndhF_p3 ACTTCCGAAACAAAGGGGAC TCAATGGTAACTTGGTCATCTCAT 50.0 37.5 58.0 57.9 2963-2980 bp 0.9 48.3
ndhF_p4 ACTTCCGAAACAAAGGGGAC TGGTAACTTGGTCATCTCATTTGA 50.0 37.5 58.0 58.1 2959-2976 bp 0.9 47.8
ndhF_p5 GGGAAAGCGAGGAAGAAACA CAATGGTAACTTGGTCATCTCA 50.0 40.9 58.1 56.1 3000 bp 0.6 31.9