Hotspot

ndhA

SSC · 125939-128222 · 2284 bp

Pi 0.00484

MarkerSeek score 44.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2284
variable_sites: 43
pi: 0.00484
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	GTTTGAGACAAGGCGCAAAT	TGGGAGAAGTTGATCGTTGA	45.0	45.0	57.6	56.5	2318-2343 bp	1.0	67.3
ndhA_p2	GTTTGAGACAAGGCGCAAAT	TGGGAGAAGTTGATCGTTGAA	45.0	42.9	57.6	57.2	2318-2343 bp	1.0	65.3
ndhA_p3	TGAGACAAGGCGCAAATAGA	TGGGAGAAGTTGATCGTTGA	45.0	45.0	57.2	56.5	2315-2340 bp	1.0	64.6
ndhA_p4	GTTTGAGACAAGGCGCAAAT	ATGGGAGAAGTTGATCGTTGA	45.0	42.9	57.6	57.0	2319-2344 bp	1.0	63.5
ndhA_p5	TGTTTGAGACAAGGCGCAAA	TGGGAGAAGTTGATCGTTGA	45.0	45.0	58.9	56.5	2319-2344 bp	1.0	63.5