Hotspot

ycf1-ndhF

SSC · 115995-116182 · 188 bp

Back to Carya

Pi 0.0739
MarkerSeek score 63.90
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
188
variable_sites
62
pi
0.0739
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 ACTTCCGAAACAAAGGGGAC ATAGGTGGTGGCCGAATTTC 50.0 50.0 58.0 57.9 588-605 bp 0.9 87.4
ycf1-ndhF_p2 ACTTCCGAAACAAAGGGGAC TCGTATCCCCATGAATCGGA 50.0 50.0 58.0 58.0 1113-1130 bp 0.9 86.9
ycf1-ndhF_p3 GGGAAAGCGAGGAAGAAACA ATAGGTGGTGGCCGAATTTC 50.0 50.0 58.1 57.9 626-643 bp 0.9 86.7
ycf1-ndhF_p4 GGGAAAGCGAGGAAGAAACA TCGTATCCCCATGAATCGGA 50.0 50.0 58.1 58.0 1151-1168 bp 0.9 86.2
ycf1-ndhF_p5 ACTTCCGAAACAAAGGGGAC CCCCATGAATCGGATAGTGC 50.0 55.0 58.0 58.2 1107-1124 bp 0.8 71.2