Hotspot

rrn5S-trnR-ACG

IRb · 113572-113812 · 241 bp

Back to Carya

Pi 0.0024
MarkerSeek score 43.50
Primer available yes
Label rrn5S-trnR-ACG

Feature Metadata

label_name
rrn5S-trnR-ACG
feature_type
igs
region
IRb
length_bp
241
variable_sites
2
pi
0.0024
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn5S-trnR-ACG_p1 GGTGACGATACTGTAGGGGA CCACGTGCTCTAATCCTCTG 55.0 55.0 57.9 57.8 332-334 bp 0.9 74.7
rrn5S-trnR-ACG_p2 AATCCATCCCGAACTTGGTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 367-369 bp 0.9 74.1
rrn5S-trnR-ACG_p3 ACCAATCCATCCCGAACTTG CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 370-372 bp 0.9 74.1
rrn5S-trnR-ACG_p4 CAATCCATCCCGAACTTGGT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 368-370 bp 0.9 74.1
rrn5S-trnR-ACG_p5 CACCAATCCATCCCGAACTT CCACGTGCTCTAATCCTCTG 50.0 55.0 57.9 57.8 371-373 bp 0.9 74.1