Hotspot

psbE-petL

LSC · 69999-71428 · 1430 bp

Back to Carya

Pi 0.00552
MarkerSeek score 60.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1430
variable_sites
28
pi
0.00552
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AACCGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.6 1459-1531 bp 1.0 72.9
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC ACCGCTAGTAGAAAACCGAA 50.0 45.0 57.8 56.6 1458-1530 bp 1.0 72.9
psbE-petL_p3 ACGAATAACCAACCCGCAAT AACCGCTAGTAGAAAACCGA 45.0 45.0 58.2 56.6 1545-1617 bp 1.0 72.5
psbE-petL_p4 ACGAATAACCAACCCGCAAT ACCGCTAGTAGAAAACCGAA 45.0 45.0 58.2 56.6 1544-1616 bp 1.0 72.5
psbE-petL_p5 TGCTGACGAATAACCAACCC ACCGCTAGTAGAAAACCGAA 50.0 45.0 58.2 56.6 1549-1621 bp 1.0 72.4