Hotspot

ndhC-trnV-UAC

LSC · 55787-56382 · 596 bp

Back to Carya

Pi 0.013
MarkerSeek score 64.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
596
variable_sites
27
pi
0.013
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCCGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 711-727 bp 1.0 84.3
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 723-739 bp 1.0 82.6
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCCGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 710-726 bp 1.0 81.5
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCCGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 699-715 bp 1.0 80.5
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 722-738 bp 1.0 79.9