Hotspot

psbC-trnS-UGA

LSC · 39472-39712 · 241 bp

Back to Carya

Pi 0.0195
MarkerSeek score 51.30
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
241
variable_sites
11
pi
0.0195
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TTATGGCATGCAGGAAGAGC GGTTGATAGCTCCGGTCTTG 50.0 55.0 58.3 58.1 412-418 bp 1.0 80.3
psbC-trnS-UGA_p2 GCTGCAGCAGGATTTGAAAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.6 58.1 385-391 bp 1.0 79.5
psbC-trnS-UGA_p3 TTATGGCATGCAGGAAGAGC GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.3 58.2 358-364 bp 1.0 79.5
psbC-trnS-UGA_p4 GCTGCAGCAGGATTTGAAAA GGGTTCGAATCCCTCTCTCT 45.0 55.0 57.6 58.2 331-337 bp 1.0 78.8
psbC-trnS-UGA_p5 TTATGGCATGCAGGAAGAGC TGGTTGATAGCTCCGGTCTT 50.0 50.0 58.3 58.4 413-419 bp 1.0 77.3