Hotspot

trnS-GCU-trnS-CGA

LSC · 9284-10132 · 849 bp

Back to Carya

Pi 0.00537
MarkerSeek score 59.60
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
849
variable_sites
18
pi
0.00537
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA GGAACGAATCACACTTTTACCA 50.0 40.9 58.0 56.9 871-971 bp 1.0 60.8
trnS-GCU-trnS-CGA_p2 CAATCCGACGCTTTAGTCCA TGGAACGAATCACACTTTTACC 50.0 40.9 58.0 56.9 872-972 bp 1.0 60.8
trnS-GCU-trnS-CGA_p3 AATCCGACGCTTTAGTCCAC GGAACGAATCACACTTTTACCA 50.0 40.9 58.0 56.9 870-970 bp 1.0 60.8
trnS-GCU-trnS-CGA_p4 AATCCGACGCTTTAGTCCAC TGGAACGAATCACACTTTTACC 50.0 40.9 58.0 56.9 871-971 bp 1.0 60.8
trnS-GCU-trnS-CGA_p5 ACAAAAGCGGAAAGAGAGGG GGAACGAATCACACTTTTACCA 50.0 40.9 58.1 56.9 931-1031 bp 1.0 60.0