Hotspot

trnK-UUU-trnQ-UUG

LSC · 6454-7829 · 1376 bp

Back to Carya

Pi 0.00572
MarkerSeek score 48.50
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1376
variable_sites
28
pi
0.00572
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AATGGCGGATGTCAGAATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1628-1685 bp 1.0 84.4
trnK-UUU-trnQ-UUG_p2 AATGGCGGATGTCAGAATCC AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.0 58.1 2279-2334 bp 1.0 83.3
trnK-UUU-trnQ-UUG_p3 AATGGCGGATGTCAGAATCC CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.0 57.9 2284-2339 bp 1.0 83.3
trnK-UUU-trnQ-UUG_p4 GATTCCCGCGTGATACACTT AGCAGCTTGCCAAACAAAAG 50.0 45.0 58.1 58.1 2599-2661 bp 1.0 82.9
trnK-UUU-trnQ-UUG_p5 GATTCCCGCGTGATACACTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 2604-2666 bp 1.0 82.9