Hotspot

ndhF-rpl32

SSC · 112835-113845 · 1011 bp

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Pi 0.0504
MarkerSeek score 80.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1011
variable_sites
48
pi
0.0504
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AAGCGGGTCGATCAAGTAAC AATTCCAGGCTTTTGTCGGT 50.0 45.0 58.0 58.0 1966 bp 0.5 59.9
ndhF-rpl32_p2 AAGCGGGTCGATCAAGTAAC ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 1965 bp 0.5 59.9
ndhF-rpl32_p3 CCGTGTGAACCAAAACCCTA AATTCCAGGCTTTTGTCGGT 50.0 45.0 58.0 58.0 2194 bp 0.5 59.7
ndhF-rpl32_p4 CCGTGTGAACCAAAACCCTA ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 2193 bp 0.5 59.7
ndhF-rpl32_p5 GTTGCCGACGGAAAAAGAAG ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 1787 bp 0.5 59.7