Hotspot

psbE-petL

LSC · 66102-67110 · 1009 bp

Back to Carum

Pi 0.045
MarkerSeek score 76.60
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1009
variable_sites
45
pi
0.045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1161-1167 bp 1.0 83.3
psbE-petL_p2 ACTGACGAATAACCAACCCG GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.9 58.0 1174-1180 bp 1.0 83.0
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1164-1170 bp 1.0 82.7
psbE-petL_p4 AGAACGTTCTCCTGTGCTTC GGTTACCGTTAAAGCCGCTA 50.0 50.0 57.8 58.0 1084-1090 bp 1.0 82.7
psbE-petL_p5 ACTGACGAATAACCAACCCG TGAGGTTACCGTTAAAGCCG 50.0 50.0 57.9 57.9 1177-1183 bp 1.0 82.4