Hotspot

petA-psbJ

LSC · 64371-65326 · 956 bp

Back to Carum

Pi 0.0725
MarkerSeek score 80.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
956
variable_sites
45
pi
0.0725
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.65
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CTTACGTGTCCAAGGCCTTT GCTGGTATTCTTGTGATCGGT 50.0 47.6 58.1 58.1 842-1146 bp 1.0 78.0
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC GCTGGTATTCTTGTGATCGGT 50.0 47.6 57.8 58.1 829-1133 bp 1.0 77.7
petA-psbJ_p3 CTTACGTGTCCAAGGCCTTT AGCTGGTATTCTTGTGATCGG 50.0 47.6 58.1 57.8 843-1147 bp 1.0 77.3
petA-psbJ_p4 GGCCTTTTGTTCTTCTTGGC AGCTGGTATTCTTGTGATCGG 50.0 47.6 57.8 57.8 830-1134 bp 1.0 76.9
petA-psbJ_p5 GATCCCTTACGTGTCCAAGG GCTGGTATTCTTGTGATCGGT 55.0 47.6 57.7 58.1 847-1151 bp 1.0 76.4