Hotspot

rbcL-accD

LSC · 58118-58765 · 648 bp

Back to Carum

Pi 0.0397
MarkerSeek score 76.50
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
648
variable_sites
24
pi
0.0397
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCTGCTGCTTGTGAAGTATG CACACCTGTGTTCGAACTCT 50.0 50.0 57.5 57.8 740-769 bp 1.0 80.4
rbcL-accD_p2 GCTGCTGCTTGTGAAGTATG TAGACCACACCTGTGTTCGA 50.0 50.0 57.5 58.3 745-774 bp 1.0 79.8
rbcL-accD_p3 ACTAAATGGAGCCCCGAACT CACACCTGTGTTCGAACTCT 50.0 50.0 58.7 57.8 761-790 bp 1.0 79.0
rbcL-accD_p4 ACTAAATGGAGCCCCGAACT TAGACCACACCTGTGTTCGA 50.0 50.0 58.7 58.3 766-795 bp 1.0 78.3
rbcL-accD_p5 GCTGCTGCTTGTGAAGTATG TTAGACCACACCTGTGTTCG 50.0 50.0 57.5 57.5 746-775 bp 1.0 78.1