Hotspot

trnE-UUC-trnT-GGU

LSC · 31763-32509 · 747 bp

Back to Carum

Pi 0.114
MarkerSeek score 83.00
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
747
variable_sites
79
pi
0.114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CCGTGTTGACAGAAAAGGGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 58.0 57.9 1255 bp 0.5 58.9
trnE-UUC-trnT-GGU_p2 TCCCGTGTTGACAGAAAAGG TACCGCTGAGTTAAAAGGGC 50.0 50.0 58.0 57.9 1257 bp 0.5 58.9
trnE-UUC-trnT-GGU_p3 GGGGGTTTAATTGGGGGTTT TACCGCTGAGTTAAAAGGGC 50.0 50.0 58.0 57.9 907 bp 0.5 58.8
trnE-UUC-trnT-GGU_p4 CTGTTCCCGTGTTGACAGAA TACCGCTGAGTTAAAAGGGC 50.0 50.0 58.1 57.9 1261 bp 0.5 58.8
trnE-UUC-trnT-GGU_p5 ATTCCTGTTCCCGTGTTGAC TACCGCTGAGTTAAAAGGGC 50.0 50.0 58.1 57.9 1265 bp 0.5 58.3