Hotspot

petN-psbM

LSC · 29357-30506 · 1150 bp

Back to Carum

Pi 0.0498
MarkerSeek score 84.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1150
variable_sites
55
pi
0.0498
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 1310-1324 bp 1.0 74.2
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 1249-1263 bp 1.0 72.9
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT AGTTCCTACTGCTTTTCTGCT 50.0 42.9 58.2 57.2 1289-1303 bp 1.0 71.3
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 1310-1324 bp 1.0 70.0
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT AGTTCCTACTGCTTTTCTGCT 50.0 42.9 57.6 57.2 1228-1242 bp 1.0 70.0