Hotspot

trnK-UUU-rps16

LSC · 4340-4870 · 531 bp

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Pi 0.0952
MarkerSeek score 79.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
531
variable_sites
46
pi
0.0952
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG CTCAGCCCACAAGAACTGTT 55.0 50.0 58.0 58.0 716-887 bp 1.0 88.6
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1411 bp 0.5 59.8
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1410 bp 0.5 59.8
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG CGATTCAAAGTGTATCGCGC 55.0 50.0 58.0 58.0 1410 bp 0.5 59.8
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG GATTCAAAGTGTATCGCGCG 55.0 50.0 58.0 58.0 1409 bp 0.5 59.8