primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	CCCCGCCGAGATTGAAAATA	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.056950	491	1552	1227-1227	0.500000	59.5
psbA_p2	psbA	2	CCTTCCTTCCAGGGTCTGTA	AAATTCGTGTGCTTGGGAGT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.066122	491	1552	1349-1349	0.500000	59.5
psbA_p3	psbA	3	TCCAGGGTCTGTAATCCGTT	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.071759	491	1552	1342-1342	0.500000	59.4
psbA_p4	psbA	4	TTCCAGGGTCTGTAATCCGT	AAATTCGTGTGCTTGGGAGT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.071759	491	1552	1343-1343	0.500000	59.4
psbA_p5	psbA	5	CCCCGCCGAGATTGAAAATA	TGCTTGGGAGTCCCTGATAA	20	20	50.0	50.0	58.0	58.0	0.0	28.7	0.072792	491	1552	1218-1218	0.500000	59.4
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	CAGACGGAAACTAAGCTCCC	AAGGCGGTTTGGACTGATTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016164	1553	1778	1274-1274	0.500000	59.9
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	CCAGACGGAAACTAAGCTCC	AAGGCGGTTTGGACTGATTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016164	1553	1778	1275-1275	0.500000	59.9
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	CCCAGACGGAAACTAAGCTC	AAGGCGGTTTGGACTGATTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016164	1553	1778	1276-1276	0.500000	59.9
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	CGGAAACTAAGCTCCCACTC	AAGGCGGTTTGGACTGATTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.016164	1553	1778	1270-1270	0.500000	59.9
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	AGCAGCAATGAAGGCGATAA	AAGGCGGTTTGGACTGATTT	20	20	45.0	45.0	58.0	58.0	0.0	0.0	0.047310	1553	1778	1028-1028	0.500000	59.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCTTTCAGGATCAGTCGTGG	CTCAGCCCACAAGAACTGTT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.042864	4340	4870	716-887	1.000000	88.6
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CCCTTTCAGGATCAGTCGTG	CGATTCAAAGTGTATCGCGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.023911	4340	4870	1411-1411	0.500000	59.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCCTTTCAGGATCAGTCGTG	GATTCAAAGTGTATCGCGCG	20	20	55.0	50.0	58.0	58.0	0.0	26.8	0.023911	4340	4870	1410-1410	0.500000	59.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCTTTCAGGATCAGTCGTGG	CGATTCAAAGTGTATCGCGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.023911	4340	4870	1410-1410	0.500000	59.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCAGGATCAGTCGTGG	GATTCAAAGTGTATCGCGCG	20	20	55.0	50.0	58.0	58.0	0.0	26.8	0.023911	4340	4870	1409-1409	0.500000	59.8
atpF-atpH_p1	atpF-atpH	1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.368120	13174	13488	406-431	1.000000	84.3
atpF-atpH_p2	atpF-atpH	2	CCAGTGGCCTAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	20	20	50.0	50.0	57.8	58.2	3.3	0.0	0.428719	13174	13488	379-404	1.000000	84.0
atpF-atpH_p3	atpF-atpH	3	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	20	20	50.0	50.0	57.9	57.6	0.0	3.3	0.536681	13174	13488	408-433	1.000000	82.9
atpF-atpH_p4	atpF-atpH	4	ATTAAACCCGAAACTCCCGG	TGAGGCGGAGGGAAAAATAC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.531623	13174	13488	477-502	1.000000	82.5
atpF-atpH_p5	atpF-atpH	5	CCAGTGGCCTAAAGAAACGA	TGAGGCGGAGGGAAAAATAC	20	20	50.0	50.0	57.8	57.6	3.3	0.0	0.592222	13174	13488	450-475	1.000000	82.2
trnC-GCA-petN_p1	trnC-GCA-petN	1	CGATATGGCCGAGTGGTAAG	AGCCCAAGCGAGACTTACTA	20	20	55.0	50.0	57.9	58.2	0.0	0.0	0.217341	28569	29266	657-794	1.000000	85.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28569	29266	617-754	1.000000	85.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	CGATATGGCCGAGTGGTAAG	AGAGTCCACTTCTTCCCCAT	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.416855	28569	29266	718-855	1.000000	83.6
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	28569	29266	678-815	1.000000	83.6
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28569	29266	635-772	1.000000	83.2
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	29357	30506	1310-1324	1.000000	74.2
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	29357	30506	1249-1263	1.000000	72.9
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	58.2	57.2	0.0	0.0	1.930302	29357	30506	1289-1303	1.000000	71.3
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	29357	30506	1310-1324	1.000000	70.0
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	AGTTCCTACTGCTTTTCTGCT	20	21	50.0	42.9	57.6	57.2	0.0	0.0	2.129816	29357	30506	1228-1242	1.000000	70.0
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CCGTGTTGACAGAAAAGGGA	CAATGAGCAACGATGGAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066626	31603	31689	522-522	0.500000	59.5
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	TCCCGTGTTGACAGAAAAGG	CAATGAGCAACGATGGAGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.066626	31603	31689	524-524	0.500000	59.5
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CCGTGTTGACAGAAAAGGGA	AATGAGCAACGATGGAGGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068045	31603	31689	521-521	0.500000	59.5
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	TCCCGTGTTGACAGAAAAGG	AATGAGCAACGATGGAGGAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.068045	31603	31689	523-523	0.500000	59.5
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	CTGTTCCCGTGTTGACAGAA	CAATGAGCAACGATGGAGGA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.080316	31603	31689	528-528	0.500000	59.4
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CCGTGTTGACAGAAAAGGGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.141744	31763	32509	1255-1255	0.500000	58.9
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TCCCGTGTTGACAGAAAAGG	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.141744	31763	32509	1257-1257	0.500000	58.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GGGGGTTTAATTGGGGGTTT	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.151306	31763	32509	907-907	0.500000	58.8
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CTGTTCCCGTGTTGACAGAA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.155433	31763	32509	1261-1261	0.500000	58.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	ATTCCTGTTCCCGTGTTGAC	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.211328	31763	32509	1265-1265	0.500000	58.3
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TGTTTGTTGTCCCGACCATT	TATTCGCGCCAGACCTAAAC	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.129483	45751	46592	1579-1760	1.000000	86.6
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	CCCGATCCCAAATCATGTGT	TATTCGCGCCAGACCTAAAC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.126570	45751	46592	1444-1444	0.500000	59.0
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	CCCGATCCCAAATCATGTGT	CCCTCGGTAAACAAAAGCCT	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.151500	45751	46592	1097-1097	0.500000	58.8
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	CCCGATCCCAAATCATGTGT	TGGGTATGGCTCCGACTATT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.154247	45751	46592	1740-1740	0.500000	58.8
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	CCCGATCCCAAATCATGTGT	TTGGGTATGGCTCCGACTAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.154247	45751	46592	1741-1741	0.500000	58.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48039	48770	782-875	1.000000	86.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48039	48770	814-907	1.000000	84.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ATTAAGTCCGCAGCGTCTAC	20	20	55.0	50.0	57.9	57.8	0.0	15.0	0.338047	48039	48770	800-893	1.000000	84.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ATTAAGTCCGCAGCGTCTAC	20	20	50.0	50.0	57.8	57.8	15.5	15.0	0.445800	48039	48770	832-925	1.000000	83.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATGCGATGCTCTAACCTCTG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.527459	48039	48770	784-877	1.000000	82.9
rbcL-accD_p1	rbcL-accD	1	GCTGCTGCTTGTGAAGTATG	CACACCTGTGTTCGAACTCT	20	20	50.0	50.0	57.5	57.8	8.5	27.3	0.764414	58118	58765	740-769	1.000000	80.4
rbcL-accD_p2	rbcL-accD	2	GCTGCTGCTTGTGAAGTATG	TAGACCACACCTGTGTTCGA	20	20	50.0	50.0	57.5	58.3	8.5	0.0	0.844502	58118	58765	745-774	1.000000	79.8
rbcL-accD_p3	rbcL-accD	3	ACTAAATGGAGCCCCGAACT	CACACCTGTGTTCGAACTCT	20	20	50.0	50.0	58.7	57.8	0.0	27.3	0.938820	58118	58765	761-790	1.000000	79.0
rbcL-accD_p4	rbcL-accD	4	ACTAAATGGAGCCCCGAACT	TAGACCACACCTGTGTTCGA	20	20	50.0	50.0	58.7	58.3	0.0	0.0	1.018908	58118	58765	766-795	1.000000	78.3
rbcL-accD_p5	rbcL-accD	5	GCTGCTGCTTGTGAAGTATG	TTAGACCACACCTGTGTTCG	20	20	50.0	50.0	57.5	57.5	8.5	0.0	1.058723	58118	58765	746-775	1.000000	78.1
petA-psbJ_p1	petA-psbJ	1	CTTACGTGTCCAAGGCCTTT	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	58.1	58.1	17.6	0.0	1.201437	64371	65326	842-1146	1.000000	78.0
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTGGC	GCTGGTATTCTTGTGATCGGT	20	21	50.0	47.6	57.8	58.1	0.0	0.0	1.248500	64371	65326	829-1133	1.000000	77.7
petA-psbJ_p3	petA-psbJ	3	CTTACGTGTCCAAGGCCTTT	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	58.1	57.8	17.6	0.0	1.293037	64371	65326	843-1147	1.000000	77.3
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTGGC	AGCTGGTATTCTTGTGATCGG	20	21	50.0	47.6	57.8	57.8	0.0	0.0	1.340100	64371	65326	830-1134	1.000000	76.9
petA-psbJ_p5	petA-psbJ	5	GATCCCTTACGTGTCCAAGG	GCTGGTATTCTTGTGATCGGT	20	21	55.0	47.6	57.7	58.1	7.7	0.0	1.404770	64371	65326	847-1151	1.000000	76.4
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	6.4	0.0	0.060086	66102	67110	1161-1167	1.000000	83.3
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.097701	66102	67110	1174-1180	1.000000	83.0
psbE-petL_p3	psbE-petL	3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	6.4	0.0	0.134501	66102	67110	1164-1170	1.000000	82.7
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	20	20	50.0	50.0	57.8	58.0	24.9	0.0	0.165095	66102	67110	1084-1090	1.000000	82.7
psbE-petL_p5	psbE-petL	5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.172116	66102	67110	1177-1183	1.000000	82.4
rpl22-rps19_p1	rpl22-rps19	1	GGACCGTCCACGAATTTGAT	AAATTTCCGAGGACACGCAA	20	20	50.0	45.0	58.3	58.4	13.0	0.0	0.665814	85139	85201	223-226	1.000000	76.1
rpl22-rps19_p2	rpl22-rps19	2	GGACCGTCCACGAATTTGAT	AATTTCCGAGGACACGCAAA	20	20	50.0	45.0	58.3	58.4	13.0	0.0	0.665814	85139	85201	222-225	1.000000	76.1
rpl22-rps19_p3	rpl22-rps19	3	GGACCGTCCACGAATTTGAT	ATTTCCGAGGACACGCAAAA	20	20	50.0	45.0	58.3	58.4	13.0	0.0	0.665814	85139	85201	221-224	1.000000	76.1
rpl22-rps19_p4	rpl22-rps19	4	GGACCGTCCACGAATTTGAT	TAAATTTCCGAGGACACGCA	20	20	50.0	45.0	58.3	57.5	13.0	0.0	0.724898	85139	85201	224-227	1.000000	75.6
rpl22-rps19_p5	rpl22-rps19	5	TAGGACCGTCCACGAATTTG	ATTTCCGAGGACACGCAAAA	20	20	50.0	45.0	57.6	58.4	19.9	0.0	0.773517	85139	85201	223-226	1.000000	75.2
ndhF-rpl32_p1	ndhF-rpl32	1	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	112835	113845	1966-1966	0.500000	59.9
ndhF-rpl32_p2	ndhF-rpl32	2	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.010594	112835	113845	1965-1965	0.500000	59.9
ndhF-rpl32_p3	ndhF-rpl32	3	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	112835	113845	2194-2194	0.500000	59.7
ndhF-rpl32_p4	ndhF-rpl32	4	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034532	112835	113845	2193-2193	0.500000	59.7
ndhF-rpl32_p5	ndhF-rpl32	5	GTTGCCGACGGAAAAAGAAG	ATTCCAGGCTTTTGTCGGTT	20	20	50.0	45.0	58.0	58.0	20.5	0.0	0.037038	112835	113845	1787-1787	0.500000	59.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TATGGGGCAGCCTTAAAAGC	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.232445	114008	114919	992-1031	1.000000	87.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TATGGGGCAGCCTTAAAAGC	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.233816	114008	114919	993-1032	1.000000	87.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TATGGGGCAGCCTTAAAAGC	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.304697	114008	114919	1013-1052	1.000000	86.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TATGGGGCAGCCTTAAAAGC	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.304697	114008	114919	1015-1054	1.000000	86.8
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TATGGGGCAGCCTTAAAAGC	CCAGAAGTGCTACTGCGAAT	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.240351	114008	114919	1621-1662	1.000000	85.4