Hotspot

rpl16

LSC · 80799-82230 · 1432 bp

Back to Carthamus

Pi 0.00504

MarkerSeek score 39.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1432
variable_sites: 13
pi: 0.00504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 39.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATGTTTGGGACTGCGTCTTT	TGACGGAAAAGAAATTGCGC	45.0	45.0	58.0	58.0	2002-2013 bp	1.0	80.9
rpl16_p2	CGCACCAAAAACTCGAGTTC	TGACGGAAAAGAAATTGCGC	50.0	45.0	58.0	58.0	2156-2167 bp	1.0	80.8
rpl16_p3	ACACATCAATTCTCGAGCCC	TGACGGAAAAGAAATTGCGC	50.0	45.0	58.0	58.0	1916-1927 bp	1.0	80.8
rpl16_p4	TCGACGATTGCTAGCTCCTA	TGACGGAAAAGAAATTGCGC	50.0	45.0	58.0	58.0	1946-1957 bp	1.0	80.7
rpl16_p5	CGCACCAAAAACTCGAGTTC	CGTCGAGGGAATTGCACATA	50.0	50.0	58.0	58.1	2492-2503 bp	1.0	80.5