Hotspot

trnL-UAG-rpl32

SSC · 123275-124117 · 843 bp

Back to Carthamus

Pi 0.00752
MarkerSeek score 54.50
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
843
variable_sites
11
pi
0.00752
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 975-1003 bp 1.0 78.0
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA AAGGAAAGGATATTGGGCGG 50.0 50.0 58.0 57.6 976-1004 bp 1.0 78.0
trnL-UAG-rpl32_p3 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 958-986 bp 1.0 77.8
trnL-UAG-rpl32_p4 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 959-987 bp 1.0 77.8
trnL-UAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA AAGGAAAGGATATTGGGCGG 50.0 50.0 57.9 57.6 985-1013 bp 1.0 77.0