Hotspot

ycf1-rps15

SSC · 113605-114018 · 414 bp

Back to Carthamus

Pi 0.0109

MarkerSeek score 39.80

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 414
variable_sites: 9
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AATAATGGGAGCCGCTTGAG	ACGCGTTACATAGGGCAAAT	50.0	45.0	58.0	58.0	1039 bp	1.0	80.8
ycf1-rps15_p2	AATAATGGGAGCCGCTTGAG	AACACCATGCATTGATGGGT	50.0	45.0	58.0	58.1	907 bp	1.0	80.6
ycf1-rps15_p3	GGAACGTGCCCATCTATCTG	ACGCGTTACATAGGGCAAAT	55.0	45.0	58.1	58.0	1508 bp	1.0	79.7
ycf1-rps15_p4	GGAACGTGCCCATCTATCTG	AACACCATGCATTGATGGGT	55.0	45.0	58.1	58.1	1376 bp	1.0	79.5
ycf1-rps15_p5	AATAATGGGAGCCGCTTGAG	AACGCGTTACATAGGGCAAA	50.0	45.0	58.0	58.2	1040 bp	1.0	79.5