Hotspot

rrn23-rrn4.5

IRb · 106642-106740 · 99 bp

Back to Carthamus

Pi 0.0118

MarkerSeek score 51.00

Primer available yes

Label rrn23-rrn4.5

Feature Metadata

label_name: rrn23-rrn4.5
feature_type: igs
region: IRb
length_bp: 99
variable_sites: 2
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23-rrn4.5_p1	AAGTAGTAAGCCCACCCCAA	TCACTGCACTTCCACTTGAC	50.0	50.0	58.2	58.0	194 bp	1.0	78.5
rrn23-rrn4.5_p2	AAGTAGTAAGCCCACCCCAA	CTGTTAGGATGCCTCAGCTG	50.0	55.0	58.2	58.0	220 bp	1.0	78.3
rrn23-rrn4.5_p3	AAGTAGTAAGCCCACCCCAA	GTCTGTTAGGATGCCTCAGC	50.0	55.0	58.2	58.1	222 bp	1.0	78.3
rrn23-rrn4.5_p4	TAGTAAGCCCACCCCAAGAT	TCACTGCACTTCCACTTGAC	50.0	50.0	57.7	58.0	191 bp	1.0	78.2
rrn23-rrn4.5_p5	TAGTAAGCCCACCCCAAGAT	CTGTTAGGATGCCTCAGCTG	50.0	55.0	57.7	58.0	217 bp	1.0	78.1