Hotspot

clpP-psbB

LSC · 71633-72076 · 444 bp

Back to Carthamus

Pi 0.00715

MarkerSeek score 51.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 444
variable_sites: 6
pi: 0.00715
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGATGCATCTTCCTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.3	58.2	526-530 bp	1.0	73.4
clpP-psbB_p2	ACCCAAGATGCATCTTCCTC	AGCAACCAAAGCTGTATGCA	50.0	45.0	57.3	58.4	599-603 bp	1.0	72.1
clpP-psbB_p3	TGTCAACCCAAGATGCATCT	CGATACCAAGGCAAACCCAT	45.0	50.0	57.1	58.2	531-535 bp	1.0	72.1
clpP-psbB_p4	ACCCAAGATGCATCTTCCTC	ACACGATACCAAGGCAAACC	50.0	50.0	57.3	58.5	529-533 bp	1.0	71.5
clpP-psbB_p5	AACCCAAGATGCATCTTCCTC	CGATACCAAGGCAAACCCAT	47.6	50.0	58.0	58.2	527-531 bp	1.0	71.1