Hotspot

trnT-UGU-trnL-UAA

LSC · 46049-46587 · 539 bp

Back to Carthamus

Pi 0.0102

MarkerSeek score 42.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 539
variable_sites: 10
pi: 0.0102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CTAACCTCTGAGCTAAGCAGG	ACCAATTTCGCCATATCCCC	52.4	50.0	57.5	58.0	579-581 bp	1.0	70.0
trnT-UGU-trnL-UAA_p2	CTAACCTCTGAGCTAAGCAGG	TAGCGTCTACCAATTTCGCC	52.4	50.0	57.5	58.1	587-589 bp	1.0	69.5
trnT-UGU-trnL-UAA_p3	TAACCTCTGAGCTAAGCAGG	ACCAATTTCGCCATATCCCC	50.0	50.0	56.3	58.0	578-580 bp	1.0	68.4
trnT-UGU-trnL-UAA_p4	CTAACCTCTGAGCTAAGCAGG	AGTCCGTAGCGTCTACCAAT	52.4	50.0	57.5	58.2	593-595 bp	1.0	68.1
trnT-UGU-trnL-UAA_p5	TAACCTCTGAGCTAAGCAGG	TAGCGTCTACCAATTTCGCC	50.0	50.0	56.3	58.1	586-588 bp	1.0	67.9