Hotspot

trnE-UUC-rpoB

LSC · 11703-12653 · 951 bp

Back to Carthamus

Pi 0.00742

MarkerSeek score 50.90

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 13
pi: 0.00742
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	GAGAGAAGTCGTAACCGCTG	AGTTCTTCTGTCAAACCCTGA	55.0	42.9	58.1	56.8	1036-1063 bp	1.0	65.0
trnE-UUC-rpoB_p2	AACCCTCGTTATCTCCGTGA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	1058-1085 bp	1.0	64.5
trnE-UUC-rpoB_p3	ACCCTCGTTATCTCCGTGAA	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	1057-1084 bp	1.0	64.5
trnE-UUC-rpoB_p4	TGAACCCTCGTTATCTCCGT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	1060-1087 bp	1.0	64.5
trnE-UUC-rpoB_p5	AGAGAGAAGTCGTAACCGCT	AGTTCTTCTGTCAAACCCTGA	50.0	42.9	58.2	56.8	1037-1064 bp	1.0	64.4