Hotspot

trnS-GCU-trnC-GCA

LSC · 8554-9327 · 774 bp

Back to Carthamus

Pi 0.00589

MarkerSeek score 50.30

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 9
pi: 0.00589
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	GTTTGTTTATGAGGCGGCAC	50.0	50.0	58.2	58.0	942-945 bp	1.0	81.3
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	TCAGAGATTCTCGCGAGTCT	50.0	50.0	58.2	58.0	973-976 bp	1.0	81.2
trnS-GCU-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	922-925 bp	1.0	80.9
trnS-GCU-trnC-GCA_p4	ACGGAAAGAGAGGGATTCGA	CACTCAGAGATTCTCGCGAG	50.0	55.0	58.2	57.9	976-979 bp	1.0	80.6
trnS-GCU-trnC-GCA_p5	ACGGAAAGAGAGGGATTCGA	ACTCAGAGATTCTCGCGAGT	50.0	50.0	58.2	58.3	975-978 bp	1.0	79.4