Hotspot

trnN-GTT-rpl32

SSC · 112824-117578 · 4755 bp

Back to Carpinus

Pi 0.00337

MarkerSeek score 48.20

Primer available yes

Label trnN-GTT-rpl32

Feature Metadata

label_name: trnN-GTT-rpl32
feature_type: igs
region: SSC
length_bp: 4755
variable_sites: 40
pi: 0.00337
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GTT-rpl32_p1	ATGGAATCGCCCATTTCGAT	ATATAGGGGGTGGCCGAATT	45.0	50.0	57.7	58.2	224-348 bp	1.0	87.2
trnN-GTT-rpl32_p2	GATGGAATCGCCCATTTCGA	ATATAGGGGGTGGCCGAATT	50.0	50.0	58.4	58.2	225-349 bp	1.0	86.1
trnN-GTT-rpl32_p3	ATGGAATCGCCCATTTCGAT	GGGTGGCCGAATTTCTTCTT	45.0	50.0	57.7	58.5	217-341 bp	1.0	85.8
trnN-GTT-rpl32_p4	ATGGAATCGCCCATTTCGAT	TATAGGGGGTGGCCGAATTT	45.0	50.0	57.7	58.5	223-347 bp	1.0	85.4
trnN-GTT-rpl32_p5	GATGGAATCGCCCATTTCGA	GGGTGGCCGAATTTCTTCTT	50.0	50.0	58.4	58.5	218-342 bp	1.0	84.7