Hotspot

petA-psbJ

LSC · 66851-67957 · 1107 bp

Back to Carpinus

Pi 0.00138

MarkerSeek score 56.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1107
variable_sites: 7
pi: 0.00138
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1233-1238 bp	1.0	76.6
petA-psbJ_p2	GATCCATTACGTGCCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.7	57.6	1251-1256 bp	1.0	72.3
petA-psbJ_p3	CCCAAGGCCTTTTGTTCTTC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.2	57.6	1238-1243 bp	1.0	71.3
petA-psbJ_p4	GCCTTTTGTTCTTCTTGGCA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.1	57.6	1232-1237 bp	1.0	70.7
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	TGGTTCATATTCCGGATTGGG	50.0	47.6	57.8	57.8	1241-1246 bp	1.0	69.8