Hotspot

atpH-atpI

LSC · 15358-16566 · 1209 bp

Back to Carpinus

Pi 0.00344

MarkerSeek score 61.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1209
variable_sites: 16
pi: 0.00344
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1259-1305 bp	1.0	82.2
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1260-1306 bp	1.0	81.0
atpH-atpI_p3	ATAGAAGCAAGACCGACAGC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.7	57.9	1292-1338 bp	1.0	80.0
atpH-atpI_p4	CAGCAGTACCTTGACCAACC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.5	57.9	1321-1367 bp	1.0	78.5
atpH-atpI_p5	ATAACGGAAGCGGCAGAAAT	AGGCGAATCTATGGAGGGTC	45.0	55.0	58.0	58.7	1232-1278 bp	1.0	78.1