Hotspot

trnQ-TTG-psbK

LSC · 7973-8424 · 452 bp

Back to Carpinus

Pi 0.00737

MarkerSeek score 59.20

Primer available yes

Label trnQ-TTG-psbK

Feature Metadata

label_name: trnQ-TTG-psbK
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 18
pi: 0.00737
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-TTG-psbK_p1	ATAGGTATGCTCTGGGACGG	CGTAGGCTTCAGGCAATTTG	55.0	50.0	58.4	57.5	626-649 bp	1.0	77.2
trnQ-TTG-psbK_p2	GAATACCGGGACCAAAACCC	CGTAGGCTTCAGGCAATTTG	55.0	50.0	58.5	57.5	590-613 bp	1.0	76.1
trnQ-TTG-psbK_p3	ATAGGTATGCTCTGGGACGG	TAGGCTTCAGGCAATTTGGC	55.0	50.0	58.4	58.8	624-647 bp	1.0	75.1
trnQ-TTG-psbK_p4	GACCAAAACCCGTTGCCTTA	CGTAGGCTTCAGGCAATTTG	50.0	50.0	58.7	57.5	581-604 bp	1.0	75.0
trnQ-TTG-psbK_p5	ATAGGTATGCTCTGGGACGG	GTAGGCTTCAGGCAATTTGG	55.0	50.0	58.4	57.1	625-648 bp	1.0	74.0