Hotspot

rps16-trnQ-TTG

LSC · 6462-7900 · 1439 bp

Back to Carpinus

Pi 0.000844
MarkerSeek score 55.50
Primer available yes
Label rps16-trnQ-TTG

Feature Metadata

label_name
rps16-trnQ-TTG
feature_type
igs
region
LSC
length_bp
1439
variable_sites
6
pi
0.000844
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-TTG_p1 TCCACAACCGACCATATCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 554-1534 bp 1.0 82.7
rps16-trnQ-TTG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 532-1512 bp 1.0 82.6
rps16-trnQ-TTG_p3 TCCACAACCGACCATATCCT GGGTTTTGGTCCCGGTATTC 50.0 55.0 58.1 58.5 575-1555 bp 1.0 79.1
rps16-trnQ-TTG_p4 AAGTCGCACGTTGCTTTCTA GGGTTTTGGTCCCGGTATTC 45.0 55.0 58.1 58.5 553-1533 bp 1.0 79.0
rps16-trnQ-TTG_p5 CCACAACCGACCATATCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 553-1533 bp 1.0 78.1