Hotspot

matK-rps16

LSC · 3674-5329 · 1656 bp

Back to Carpinus

Pi 0.00285

MarkerSeek score 63.30

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1656
variable_sites: 20
pi: 0.00285
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	CCATGATCATGAGCAAGTGC	AGGCGGGAGTTTTTATGGAC	50.0	50.0	57.2	57.9	1798-2002 bp	0.916667	71.7
matK-rps16_p2	CCATGATCATGAGCAAGTGC	GCGGGAGTTTTTATGGACCT	50.0	50.0	57.2	57.9	1796-2000 bp	0.916667	71.7
matK-rps16_p3	CCATGATCATGAGCAAGTGC	TCAAAGAAGGCGGGAGTTTT	50.0	45.0	57.2	57.6	1805-2009 bp	0.916667	69.8
matK-rps16_p4	CCATGATCATGAGCAAGTGC	ACATTTCAAAGAAGGCGGGA	50.0	45.0	57.2	57.7	1810-2014 bp	0.833333	67.0
matK-rps16_p5	CCATGATCATGAGCAAGTGC	TTCAAAGAAGGCGGGAGTTT	50.0	45.0	57.2	57.6	1806-2010 bp	0.833333	66.4