Hotspot

trnH-GTG-psbA

LSC · 84-503 · 420 bp

Back to Carpinus

Pi 0.00456
MarkerSeek score 55.50
Primer available yes
Label trnH-GTG-psbA

Feature Metadata

label_name
trnH-GTG-psbA
feature_type
igs
region
LSC
length_bp
420
variable_sites
5
pi
0.00456
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GTG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 524-536 bp 1.0 81.6
trnH-GTG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 538-550 bp 1.0 81.5
trnH-GTG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 537-549 bp 1.0 81.5
trnH-GTG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 539-551 bp 1.0 81.5
trnH-GTG-psbA_p5 TATTTATGGGCGAACGACGG GCGCTAACCTTGGTATGGAA 50.0 50.0 58.1 58.0 585-597 bp 0.75 70.9