primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GTG-psbA_p1	trnH-GTG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	84	503	524-536	1.000000	81.6
trnH-GTG-psbA_p2	trnH-GTG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	84	503	538-550	1.000000	81.5
trnH-GTG-psbA_p3	trnH-GTG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	84	503	537-549	1.000000	81.5
trnH-GTG-psbA_p4	trnH-GTG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	84	503	539-551	1.000000	81.5
trnH-GTG-psbA_p5	trnH-GTG-psbA	5	TATTTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.177167	84	503	585-597	0.750000	70.9
matK-rps16_p1	matK-rps16	1	CCATGATCATGAGCAAGTGC	AGGCGGGAGTTTTTATGGAC	20	20	50.0	50.0	57.2	57.9	32.7	0.0	0.900589	3674	5329	1798-2002	0.916667	71.7
matK-rps16_p2	matK-rps16	2	CCATGATCATGAGCAAGTGC	GCGGGAGTTTTTATGGACCT	20	20	50.0	50.0	57.2	57.9	32.7	0.0	0.900589	3674	5329	1796-2000	0.916667	71.7
matK-rps16_p3	matK-rps16	3	CCATGATCATGAGCAAGTGC	TCAAAGAAGGCGGGAGTTTT	20	20	50.0	45.0	57.2	57.6	32.7	0.0	1.134633	3674	5329	1805-2009	0.916667	69.8
matK-rps16_p4	matK-rps16	4	CCATGATCATGAGCAAGTGC	ACATTTCAAAGAAGGCGGGA	20	20	50.0	45.0	57.2	57.7	32.7	0.0	1.062676	3674	5329	1810-2014	0.833333	67.0
matK-rps16_p5	matK-rps16	5	CCATGATCATGAGCAAGTGC	TTCAAAGAAGGCGGGAGTTT	20	20	50.0	45.0	57.2	57.6	32.7	0.0	1.134633	3674	5329	1806-2010	0.833333	66.4
rps16-trnQ-TTG_p1	rps16-trnQ-TTG	1	TCCACAACCGACCATATCCT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.1	58.1	0.0	16.9	0.201972	6462	7900	554-1534	1.000000	82.7
rps16-trnQ-TTG_p2	rps16-trnQ-TTG	2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6462	7900	532-1512	1.000000	82.6
rps16-trnQ-TTG_p3	rps16-trnQ-TTG	3	TCCACAACCGACCATATCCT	GGGTTTTGGTCCCGGTATTC	20	20	50.0	55.0	58.1	58.5	0.0	0.0	0.659014	6462	7900	575-1555	1.000000	79.1
rps16-trnQ-TTG_p4	rps16-trnQ-TTG	4	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGGTATTC	20	20	45.0	55.0	58.1	58.5	8.1	0.0	0.672468	6462	7900	553-1533	1.000000	79.0
rps16-trnQ-TTG_p5	rps16-trnQ-TTG	5	CCACAACCGACCATATCCTT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.3	58.1	0.0	16.9	0.786197	6462	7900	553-1533	1.000000	78.1
trnQ-TTG-psbK_p1	trnQ-TTG-psbK	1	ATAGGTATGCTCTGGGACGG	CGTAGGCTTCAGGCAATTTG	20	20	55.0	50.0	58.4	57.5	0.0	2.9	0.915860	7973	8424	626-649	1.000000	77.2
trnQ-TTG-psbK_p2	trnQ-TTG-psbK	2	GAATACCGGGACCAAAACCC	CGTAGGCTTCAGGCAATTTG	20	20	55.0	50.0	58.5	57.5	6.3	2.9	1.082777	7973	8424	590-613	1.000000	76.1
trnQ-TTG-psbK_p3	trnQ-TTG-psbK	3	ATAGGTATGCTCTGGGACGG	TAGGCTTCAGGCAATTTGGC	20	20	55.0	50.0	58.4	58.8	0.0	14.7	1.180911	7973	8424	624-647	1.000000	75.1
trnQ-TTG-psbK_p4	trnQ-TTG-psbK	4	GACCAAAACCCGTTGCCTTA	CGTAGGCTTCAGGCAATTTG	20	20	50.0	50.0	58.7	57.5	0.0	2.9	1.229126	7973	8424	581-604	1.000000	75.0
trnQ-TTG-psbK_p5	trnQ-TTG-psbK	5	ATAGGTATGCTCTGGGACGG	GTAGGCTTCAGGCAATTTGG	20	20	55.0	50.0	58.4	57.1	0.0	2.9	1.313674	7973	8424	625-648	1.000000	74.0
trnS-GCT-trnR-TCT_p1	trnS-GCT-trnR-TCT	1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	9365	11415	2027-2139	1.000000	69.7
trnS-GCT-trnR-TCT_p2	trnS-GCT-trnR-TCT	2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.057575	9365	11415	2026-2138	1.000000	69.6
trnS-GCT-trnR-TCT_p3	trnS-GCT-trnR-TCT	3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	9365	11415	2033-2145	1.000000	69.0
trnS-GCT-trnR-TCT_p4	trnS-GCT-trnR-TCT	4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.140903	9365	11415	2032-2144	1.000000	69.0
trnS-GCT-trnR-TCT_p5	trnS-GCT-trnR-TCT	5	ACAAAAGCGGAAAGAGAGGG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.1	56.9	0.0	0.0	2.154045	9365	11415	2087-2199	1.000000	68.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.0	57.9	1.4	0.0	0.171127	15358	16566	1259-1305	1.000000	82.2
atpH-atpI_p2	atpH-atpI	2	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	58.2	57.9	1.4	0.0	0.316666	15358	16566	1260-1306	1.000000	81.0
atpH-atpI_p3	atpH-atpI	3	ATAGAAGCAAGACCGACAGC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.7	57.9	0.0	0.0	0.434317	15358	16566	1292-1338	1.000000	80.0
atpH-atpI_p4	atpH-atpI	4	CAGCAGTACCTTGACCAACC	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	58.5	57.9	0.0	0.0	0.608982	15358	16566	1321-1367	1.000000	78.5
atpH-atpI_p5	atpH-atpI	5	ATAACGGAAGCGGCAGAAAT	AGGCGAATCTATGGAGGGTC	20	20	45.0	55.0	58.0	58.7	1.4	0.0	0.695318	15358	16566	1232-1278	1.000000	78.1
trnT-GGT-psbD_p1	trnT-GGT-psbD	1	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	34899	36326	1294-1606	1.000000	89.5
trnT-GGT-psbD_p2	trnT-GGT-psbD	2	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.323334	34899	36326	1308-1620	1.000000	89.4
trnT-GGT-psbD_p3	trnT-GGT-psbD	3	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	34899	36326	1297-1609	1.000000	89.3
trnT-GGT-psbD_p4	trnT-GGT-psbD	4	TCAGTGGTAGAGTAACGCCA	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	58.1	57.7	0.0	9.5	0.345488	34899	36326	1311-1623	1.000000	89.2
trnT-GGT-psbD_p5	trnT-GGT-psbD	5	GTGGTAGAGTAACGCCATGG	TAGACCGGACCAACCTACAA	20	20	55.0	50.0	58.1	57.7	7.6	9.5	0.371563	34899	36326	1310-1622	1.000000	89.0
trnT-TGT-trnF-GAA_p1	trnT-TGT-trnF-GAA	1	GCGATGCTCTAACCTCTGAG	TCTACCAGCTGAGCTATCCC	20	20	55.0	55.0	57.9	58.0	0.0	17.4	0.135246	50131	51920	1804-1843	1.000000	82.4
trnT-TGT-trnF-GAA_p2	trnT-TGT-trnF-GAA	2	AAAGCCTGCTATCGGAATCG	TCTACCAGCTGAGCTATCCC	20	20	50.0	55.0	58.1	58.0	0.0	17.4	0.131425	50131	51920	1849-1888	1.000000	82.3
trnT-TGT-trnF-GAA_p3	trnT-TGT-trnF-GAA	3	AAAGCCTGCTATCGGAATCG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	58.1	58.1	0.0	20.1	0.180816	50131	51920	1885-1924	1.000000	81.9
trnT-TGT-trnF-GAA_p4	trnT-TGT-trnF-GAA	4	GCGATGCTCTAACCTCTGAG	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.9	58.1	0.0	20.1	0.184636	50131	51920	1840-1879	1.000000	81.9
trnT-TGT-trnF-GAA_p5	trnT-TGT-trnF-GAA	5	AAAGCCTGCTATCGGAATCG	GTGCCAGGAACCAGATTTGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.219220	50131	51920	1900-1939	1.000000	81.5
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	ATGCCGGAAAGTTGGATAGG	20	20	55.0	50.0	58.0	57.7	10.3	5.0	0.350916	51994	52780	938-1318	1.000000	81.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	ATGCCGGAAAGTTGGATAGG	20	20	50.0	50.0	58.1	57.7	0.0	5.0	0.400307	51994	52780	902-1282	1.000000	80.7
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CTGGTAGAGCAGAGGACTGA	ATGCCGGAAAGTTGGATAGG	20	20	55.0	50.0	57.9	57.7	2.5	5.0	0.484709	51994	52780	926-1306	1.000000	79.9
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	AAGATAAGGATGATGCGCCG	ATGCCGGAAAGTTGGATAGG	20	20	50.0	50.0	58.2	57.7	4.2	5.0	0.547605	51994	52780	967-1347	1.000000	79.3
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGGATAGCTCAGCTGGTAGA	GCCGGAAAGTTGGATAGGAT	20	20	55.0	50.0	58.0	57.4	10.3	5.0	0.642821	51994	52780	936-1316	1.000000	78.6
ndhC-trnM-CAT_p1	ndhC-trnM-CAT	1	GGGGCTAAAACTCCCGAAAT	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.9	58.4	0.0	0.0	0.550649	54507	55926	1559-1611	1.000000	77.5
ndhC-trnM-CAT_p2	ndhC-trnM-CAT	2	CCTTTGCTAATCGGGGCTAA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.7	58.4	0.0	0.0	0.753172	54507	55926	1571-1623	1.000000	75.8
ndhC-trnM-CAT_p3	ndhC-trnM-CAT	3	GGGGCTAAAACTCCCGAAAT	CCGCCGTATGAAAGCAATAC	20	20	50.0	50.0	57.9	57.3	0.0	0.0	0.802602	54507	55926	1558-1610	1.000000	75.5
ndhC-trnM-CAT_p4	ndhC-trnM-CAT	4	GGGGCTAAAACTCCCGAAAT	TTTGAACCAATGACTCCCGC	20	20	50.0	50.0	57.9	58.8	0.0	0.0	0.895421	54507	55926	1574-1626	1.000000	74.7
ndhC-trnM-CAT_p5	ndhC-trnM-CAT	5	CTTCTCTGGCCCTTTGCTAA	CCCGCCGTATGAAAGCAATA	20	20	50.0	50.0	57.5	58.4	0.0	0.0	0.908224	54507	55926	1581-1633	1.000000	74.6
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.8	57.6	0.0	4.1	0.519826	66851	67957	1233-1238	1.000000	76.6
petA-psbJ_p2	petA-psbJ	2	GATCCATTACGTGCCCAAGG	ATTCCGGATTGGGTTCATCC	20	20	55.0	50.0	58.7	57.6	0.0	4.1	1.055974	66851	67957	1251-1256	1.000000	72.3
petA-psbJ_p3	petA-psbJ	3	CCCAAGGCCTTTTGTTCTTC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.2	57.6	17.6	4.1	1.185031	66851	67957	1238-1243	1.000000	71.3
petA-psbJ_p4	petA-psbJ	4	GCCTTTTGTTCTTCTTGGCA	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	57.1	57.6	0.0	4.1	1.253806	66851	67957	1232-1237	1.000000	70.7
petA-psbJ_p5	petA-psbJ	5	GGCCTTTTGTTCTTCTTGGC	TGGTTCATATTCCGGATTGGG	20	21	50.0	47.6	57.8	57.8	0.0	4.1	1.377027	66851	67957	1241-1246	1.000000	69.8
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	AACCGCTAGTAGAAAACCGA	20	20	50.0	45.0	57.8	56.6	24.9	0.0	1.559955	68740	69792	1122-1143	1.000000	68.2
psbE-petL_p2	psbE-petL	2	AGAACGTTCTCCTGTGCTTC	ACCGCTAGTAGAAAACCGAA	20	20	50.0	45.0	57.8	56.6	24.9	0.0	1.559955	68740	69792	1121-1142	1.000000	68.2
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AACCGCTAGTAGAAAACCGA	20	20	45.0	45.0	58.2	56.6	0.0	0.0	1.574632	68740	69792	1208-1229	1.000000	68.1
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	ACCGCTAGTAGAAAACCGAA	20	20	45.0	45.0	58.2	56.6	0.0	0.0	1.574632	68740	69792	1207-1228	1.000000	68.1
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	ACCGCTAGTAGAAAACCGAA	20	20	50.0	45.0	58.2	56.6	0.0	0.0	1.587537	68740	69792	1212-1233	1.000000	68.0
trnN-GTT-rpl32_p1	trnN-GTT-rpl32	1	ATGGAATCGCCCATTTCGAT	ATATAGGGGGTGGCCGAATT	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.521532	114318	114437	224-348	1.000000	87.2
trnN-GTT-rpl32_p2	trnN-GTT-rpl32	2	GATGGAATCGCCCATTTCGA	ATATAGGGGGTGGCCGAATT	20	20	50.0	50.0	58.4	58.2	0.0	0.0	0.651604	114318	114437	225-349	1.000000	86.1
trnN-GTT-rpl32_p3	trnN-GTT-rpl32	3	ATGGAATCGCCCATTTCGAT	GGGTGGCCGAATTTCTTCTT	20	20	45.0	50.0	57.7	58.5	0.0	0.0	0.730891	114318	114437	217-341	1.000000	85.8
trnN-GTT-rpl32_p4	trnN-GTT-rpl32	4	ATGGAATCGCCCATTTCGAT	TATAGGGGGTGGCCGAATTT	20	20	45.0	50.0	57.7	58.5	0.0	0.0	0.751557	114318	114437	223-347	1.000000	85.4
trnN-GTT-rpl32_p5	trnN-GTT-rpl32	5	GATGGAATCGCCCATTTCGA	GGGTGGCCGAATTTCTTCTT	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.860962	114318	114437	218-342	1.000000	84.7
rpl32-trnL-TAG_p1	rpl32-trnL-TAG	1	GGAGGGGTATTGGGTAGCAT	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.6	58.0	0.0	0.0	0.561815	117735	118752	1112-1157	1.000000	79.1
rpl32-trnL-TAG_p2	rpl32-trnL-TAG	2	ATGGAGGGGTATTGGGTAGC	AGCGTGTCTACCGATTTCAC	20	20	55.0	50.0	58.6	58.0	0.0	0.0	0.561815	117735	118752	1114-1159	1.000000	79.0
rpl32-trnL-TAG_p3	rpl32-trnL-TAG	3	ATGGAGGGGTATTGGGTAGC	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.6	58.0	0.0	0.0	0.563185	117735	118752	1115-1160	1.000000	79.0
rpl32-trnL-TAG_p4	rpl32-trnL-TAG	4	GGAGGGGTATTGGGTAGCAT	CAGCGTGTCTACCGATTTCA	20	20	55.0	50.0	58.6	58.0	0.0	0.0	0.563185	117735	118752	1113-1158	1.000000	79.0
rpl32-trnL-TAG_p5	rpl32-trnL-TAG	5	GGAGGGGTATTGGGTAGCAT	TCCTAAGAGCAGCGTGTCTA	20	20	55.0	50.0	58.6	57.9	0.0	0.0	0.678262	117735	118752	1122-1167	1.000000	78.1
ndhA_p1	ndhA	1	TGACCCCAGTTACCATAGGG	CCCGCCGGGTTTTATCAATT	20	20	55.0	50.0	58.1	58.9	0.0	15.9	1.004258	124454	126744	2509-2528	1.000000	72.9
ndhA_p2	ndhA	2	TGACCCCAGTTACCATAGGG	CCGCCGGGTTTTATCAATTT	20	20	55.0	45.0	58.1	57.0	0.0	2.2	1.063660	124454	126744	2508-2527	1.000000	72.4
ndhA_p3	ndhA	3	GACCCCAGTTACCATAGGGA	CCCGCCGGGTTTTATCAATT	20	20	55.0	50.0	57.8	58.9	0.0	15.9	1.077718	124454	126744	2508-2527	1.000000	72.3
ndhA_p4	ndhA	4	GACCCCAGTTACCATAGGGA	CCGCCGGGTTTTATCAATTT	20	20	55.0	45.0	57.8	57.0	0.0	2.2	1.137120	124454	126744	2507-2526	1.000000	71.9
ndhA_p5	ndhA	5	TGACCCCAGTTACCATAGGG	GCCGGGTTTTATCAATTTGCA	20	21	55.0	42.9	58.1	58.0	0.0	2.2	1.146153	124454	126744	2506-2525	1.000000	71.8