Hotspot

ycf1

SSC · 122186-127666 · 5481 bp

Back to Caroxylon

Pi 0.0162

MarkerSeek score 48.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5481
variable_sites: 132
pi: 0.0162
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTGATAAGTACTTTTTGATTTTAACCA	ACTTAAGATCTGTCCTTCAGT	25.9	38.1	55.2	53.6	170 bp	1.0	43.8
ycf1_p2	GTGATAAGTACTTTTTGATTTTAACC	ACTTAAGATCTGTCCTTCAGT	26.9	38.1	53.7	53.6	170 bp	1.0	43.8
ycf1_p3	GTGATAAGTACTTTTTGATTTTAACCA	AACTTAAGATCTGTCCTTCAGT	25.9	36.4	55.2	54.5	171 bp	1.0	43.7
ycf1_p4	AGTGATAAGTACTTTTTGATTTTAACC	ACTTAAGATCTGTCCTTCAGT	25.9	38.1	55.0	53.6	171 bp	1.0	43.7
ycf1_p5	AGTGATAAGTACTTTTTGATTTTAACC	AACTTAAGATCTGTCCTTCAGT	25.9	36.4	55.0	54.5	172 bp	1.0	43.7