Hotspot

ndhF-rpl32

SSC · 110504-111614 · 1111 bp

Back to Caroxylon

Pi 0.0255

MarkerSeek score 69.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1111
variable_sites: 42
pi: 0.0255
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTTTGTTGCGGTCGGAAAAA	TTAACGCTGCCCAATATCCC	45.0	50.0	57.8	58.0	1296-1301 bp	1.0	81.6
ndhF-rpl32_p2	CTTTGTTGCGGTCGGAAAAA	AAAAGGCTTTTAACGCTGCC	45.0	45.0	57.8	57.9	1305-1310 bp	1.0	80.5
ndhF-rpl32_p3	TGGAAGTGGAAGGAAGGGTA	TTAACGCTGCCCAATATCCC	50.0	50.0	57.6	58.0	1245-1250 bp	1.0	79.9
ndhF-rpl32_p4	TGGAAGTGGAAGGAAGGGTA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	57.6	57.9	1254-1259 bp	1.0	78.8
ndhF-rpl32_p5	TCTTTGTTGCGGTCGGAAAA	TTAACGCTGCCCAATATCCC	45.0	50.0	58.6	58.0	1297-1302 bp	1.0	78.2