Hotspot

rpoA-rps11

LSC · 78542-78612 · 71 bp

Back to Caroxylon

Pi 0.0329

MarkerSeek score 64.80

Primer available yes

Label rpoA-rps11

Feature Metadata

label_name: rpoA-rps11
feature_type: igs
region: LSC
length_bp: 71
variable_sites: 3
pi: 0.0329
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps11_p1	ACTTCCACTGTAGTGTCCGA	GGCTGTAGACCTCCTAAAAA	50.0	45.0	58.0	54.4	143-153 bp	1.0	56.5
rpoA-rps11_p2	TTCCACTGTAGTGTCCGAGT	GGCTGTAGACCTCCTAAAAA	50.0	45.0	58.0	54.4	141-151 bp	1.0	56.5
rpoA-rps11_p3	CACTTCCACTGTAGTGTCCG	GGCTGTAGACCTCCTAAAAA	55.0	45.0	57.9	54.4	144-154 bp	1.0	56.0
rpoA-rps11_p4	CTTCCACTGTAGTGTCCGAG	GGCTGTAGACCTCCTAAAAA	55.0	45.0	57.4	54.4	142-152 bp	1.0	51.5
rpoA-rps11_p5	TCCACTGTAGTGTCCGAGTA	GGCTGTAGACCTCCTAAAAA	50.0	45.0	57.1	54.4	140-150 bp	1.0	49.7