Hotspot

trnT-UGU-trnL-UAA

LSC · 46680-47383 · 704 bp

Back to Caroxylon

Pi 0.0256
MarkerSeek score 63.30
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
704
variable_sites
27
pi
0.0256
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.9 58.1 758-777 bp 1.0 83.3
trnT-UGU-trnL-UAA_p2 AGTCCAGGTAAAGCCCCTAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 58.1 58.1 1300-1315 bp 1.0 82.9
trnT-UGU-trnL-UAA_p3 GTCCAGGTAAAGCCCCTAGA AGCCAATTAAGTCCGTAGCG 55.0 50.0 58.1 58.1 1299-1314 bp 1.0 82.8
trnT-UGU-trnL-UAA_p4 TCCAGGTAAAGCCCCTAGAC AGCCAATTAAGTCCGTAGCG 55.0 50.0 58.1 58.1 1298-1313 bp 1.0 82.8
trnT-UGU-trnL-UAA_p5 AGTCCAGGTAAAGCCCCTAG GGATTGGAGTTTCGACGGAA 55.0 50.0 58.1 57.9 1523-1535 bp 1.0 82.3